David Hage
Professor Chemistry University of Nebraska-Lincoln
Contact
- Address
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HAH 704
Lincoln NE 68588-0304 - Phone
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402-472-2744 On-campus 2-2744
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dhage1@unl.edu
Research interests
Affinity-based separation of proteins or metabolites from a complex mixture is a powerful method for identifying and quantifying specific components. Such methods have additional utility for determining association constants and rate constants for protein-protein or protein-ligand interactions. Dr. Hage's laboratory has developed multiple novel and innovative methods for affinity separation and bioanalysis. For example, students have used heavy water labeling followed by protein digestion and mass spectrometry to characterize residues that preferentially react to immobilize the protein in specific conditions. The students relate the coupling through those residues to resultant activity of the protein, ensuring maximal conservation of function following immobilization. Ongoing projects in the Hage laboratory use this and other methods of chromatographic separation for forensics, immunoassays, hormone and drug detection and quantification, and other important applications.